Check for air bubbles in the flow cell. Perform lamp energy checks to see if the Deuterium lamp is failing.
are flagship UV-VIS detectors within the Prominence HPLC series, widely recognized for their high sensitivity, wide linearity, and robust performance in analytical laboratories. Whether you are a new user setting up your first HPLC method or an experienced chromatographer troubleshooting a complex separation, the is an essential resource.
190 to 700 nm (SPD-20A) / 190 to 900 nm (SPD-20AV).
Avoid sites requiring credit card information. Shimadzu provides user manuals for free as PDFs. shimadzu spd20a user manual
: Allows for the simultaneous monitoring of two different wavelengths, which is useful for analyzing complex mixtures where components have different absorption maxima. Superior Linearity
The Shimadzu SPD-20A UV-VIS detector user manual outlines procedures for achieving high-sensitivity HPLC analysis within a 190 to 700 nm range, featuring a temperature-controlled flow cell and deuterium lamp management. The documentation emphasizes essential maintenance, including lamp hours, self-diagnostics, and proper cooling requirements. Access the full instruction manual on Scribd . Shimadzu SPD-20A/20AV UV-VIS Manual | PDF - Scribd
The SPD-20A uses an internal low-pressure mercury lamp for automatic wavelength calibration in the UV region. Check for air bubbles in the flow cell
Set the required wavelength and turn on the Deuterium (D₂) or Tungsten (W) lamp.
Press the zero or autozero button to establish a clean baseline before initiating your injection run. Dual-Wavelength Mode
Operating the SPD-20A correctly is crucial for reliable results. The following points are essential: Whether you are a new user setting up
Ignoring the manual is the leading cause of preventable detector downtime.
The (and the extended wavelength variant SPD-20AV ) is a cornerstone of the Shimadzu Prominence HPLC system , renowned for its superior sensitivity, stability, and versatility in UV-Visible detection. As a high-performance UV-VIS detector, it is essential for analytical chemists, QC technicians, and laboratory personnel to understand the proper operation and maintenance outlined in the user manual to achieve reliable results.
If you need a specific section of the manual or troubleshooting help for a particular error, let me know!